Semiconductor imaging technology originally developed to scan silicon wafers for defects has been modified to zoom through the human body down to the level of a single cell and could be a game-changer for medicine. The imaging technology, developed by high-tech German optical and industrial measurement manufacturer Zeiss, plus Google algorithms, is able to zoom in and out from the scale of the whole joint down to the cellular level "just as you would with Google Maps", reducing to "a matter of weeks analyses that once took 25 years to complete," said Professor Knothe Tate of UNSW Australia.
The team is also using microtome and MRI technology to examine how movement and weight bearing affects the movement of molecules within joints, exploring the relationship between blood, bone, lymphatics and muscle. "For the first time we have the ability to go from the whole body down to how the cells are getting their nutrition and how this is all connected," said Tate. "This could open the door to as yet unknown new therapies and preventions."
The team is the first to use the system in humans. The work was done in partnership with the Cleveland Clinic, Brown and Stanford Universities, as well as Zeiss and Google to help crunch terabytes of data gathered from human hip studies.Similar research is underway at Harvard University and Heidelberg in Germany to map neural pathways and connections in the brains of mice. Numerous studies have explored molecular transport within specific tissues but there has been little research on exchange between different kinds of tissue such as cartilage and bone. Tate has already demonstrated a link between molecular transport through blood, muscle and bone, and disease status in osteoarthritic guinea pigs.
Like humans, guinea pigs develop osteoarthritis as they age. The condition is increasingly believed to be the result of a breakdown in cellular communication.
Understanding the molecular signaling and traffic between tissues could unlock a range of treatments, including physical therapies and preventative exercise routines, Tate said. Critical to this work has been the development of microscopy that allows seamless imaging of organs and tissues across length scales - centimeters at the whole-joint level down to nanometer-sized molecules - as well as the capacity to sift and analyze huge sets of data.
Tate likened using the Zeiss technology in the hipbone to Google Maps' ability to zoom down from an Earth View to Street View. "These are terabyte-sized data sets so the Google maps algorithms are helping us take this tremendous amount of information and use it effectively. They're the traffic controllers, if you like. Advanced research instrumentation provides a technological platform to answer the hardest, unanswered questions in science, opening up avenues for fundamental discoveries, the implications of which may be currently unfathomable yet which will ultimately pave the way to engineer better human health and quality of life as we age."
Presented at the Orthopedic Research Society meeting in Las Vegas.