Establishment of human gastric cancer nude mouse transplantation models has undergone three representative stages: subcutaneous transplantation of cell suspension, gastric wall seeding of cell suspension, and intra-gastric wall transplantation of histologically intact tissue, of which the fresh tissue orthotopic transplantation model has two types: the "suture method"and the "gastric bursa method".But the "suture method"or the "gastric bursa method" transplantation has many practical difficulties and disadvantages in establishing the model; for example, the tumor tissue is easy to fall off postoperatively; manipulation is complex and needs relatively high skills; there may be great blood loss during suturing, and mortality is relatively high.
A research article to be published on August 14, 2008 in the World Journal of Gastroenterology addresses this question. The research team led by Prof. Xu from Shanghai Changzheng Hospital. The present study used OB glue paste technique to establish orthtopic transplantation models by implanting SGC-7901 and MKN-45 human gastric cancer cell strains into the gastric wall of nude mice. Biological features, growth of the implanted tumors, the success rate of transplantation and the rate of auto metastasis of the two models were observed.
Observations showed that the success rate of orthotopic transplantation, rate of hepatic metastasis, rate of pulmonary metastasis, rate of peritoneal metastasis, rate of lymphocytic metastasis, rate of splenic metastasis, and the occurrence of ascites of SGC-7901 and MKN-45 models were 97% and 96%; 42.13% and 94.20%; 48.43% and 57.97%; 30.83% and 36.96%; 67.30% and 84.06%, 59.75% and 10.53%, and 47.80% and 36.96%, respectively.Although different tumor strains grew at different rates, infiltrative growth and multi-organ metastases are common features of the two models, and these features are similar to the clinical presentation of invasive metastasis. Chromosomal identification also demonstrated that both orthotopic and metastatic tumors came from the human gastric cancer implanted. This technique is an ideal means of model establishment owing to its easier manipulation, shorter operating time, less blood loss, quicker postoperative recovery, higher survival of experimental animals and avoidance of tumor falling off.